bs-0523R [Primary Antibody]
SOX2 Polyclonal Antibody
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Host: Rabbit

Target Protein: SOX2

Immunogen Range: 230-280/319

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 20674

Swiss Prot: P48432

Source: KLH conjugated synthetic peptide derived from mouse Transcription factor SOX-2

Purification: Purified by Protein A.

Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Background: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
VALIDATION IMAGES

Lane 1: mouse embryo lysates Lane 2: mouse lung lysates probed with Anti SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 32kD. Observed band size: 32kD.


Formalin-fixed and paraffin embedded human stomach labeled with Anti-SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat esophagus labeled with Anti-SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


NCCIT cells probed with Rabbit Anti-SOX2 Polyclonal Antibody (bs-0523R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H L) Alexa Fluor 488 Conjugated Secondary Antibody.