bs-1970R [Primary Antibody]
LAMP-1 Polyclonal Antibody
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Host: Rabbit

Target Protein: LAMP-1

Immunogen Range: 300-350/417

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3916

Swiss Prot: P11279

Source: KLH conjugated synthetic peptide derived from human LAMP-1

Purification: Purified by Protein A.

Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Background: Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
VALIDATION IMAGES

RSC96 cells probed with LAMP-1 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-1970R-A647) at 1:100 for 30 minutes compared to control cells (blue)and isotype control (orange).


Formalin-fixed and paraffin embedded rat kidney labeled with Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.


Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.


Image provided by One World Lab validation initiative. PC3 cells were stained with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at [1:100] in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Human U937 cells probed with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).