bs-4755R [Primary Antibody]
CD19 Polyclonal Antibody
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Host: Rabbit

Target Protein: CD19

Immunogen Range: 171-220/556

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 930

Swiss Prot: P15391

Source: KLH conjugated synthetic peptide derived from human CD19

Purification: Purified by Protein A.

Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Background: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD19 Polyclonal Antibody, Unconjugated (bs-4755R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Mouse lung and liver lysates probed with CD19 Polyclonal Antibody, unconjugated (bs-4755R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-CD19 Polyclonal Antibody, Unconjugated(bs-4755R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5)used at 1:200 dilution for 40 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded fetal mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CD19 Polyclonal Antibody, Unconjugated (bs-4755R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining


Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CD19 Polyclonal Antibody, Unconjugated (bs-4755R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining